作者= Serite科妮P。,Emami-Khoyi Arsalan, Ntshudisane Ofentse K。詹姆斯·尼古拉·C。,詹森的克里斯蒂安•范维伦Bettine, Bodill世界,考利保罗D。艾伦·维特菲尔德K。Teske Peter r . TITLE =埃德娜metabarcoding vs宏基因组:评估膳食竞争在两个河口尖嘴鱼=海洋科学前沿》杂志上体积= 10年= 2023 URL = //www.thespel.com/articles/10.雷竞技rebat3389/fmars.2023.1116741 DOI = 10.3389 /抽象fmars.2023.1116741 ISSN = 2296 - 7745 =理解濒危物种的饮食偏好可能是有用的在实施保护策略,包括栖息地的恢复、易位和人工繁殖。从粪便环境DNA(埃德娜)提供了一个非侵入性的方法,分析动物的饮食。目前,metabarcoding, pcr方法,是首选的方法分析这些数据。然而,这种方法有一定的局限性,特别是PCR偏差,从而导致高估某些类群的重要性和失败检测其他类群,因为他们不放大。与宏基因组相比,本研究metabarcoding, PCR-free方法,评估猎物的多样性项目濒危南非河口尖嘴鱼的粪便,Syngnathus watermeyeri,及其广泛分布的同类美国temminckii调查潜在的食物竞争。metabarcoding结果显示明显的差异的饮食watermeyeri和s . temminckii,前者主要消费calanoid桡足类,后者喜欢caridean虾。在每种情况下,一个单一的物种占据metabarcoding生成的序列。 Metagenomics produced more species identifications, and although the same trend was found regarding the preference of S. watermeyeri for copepods and that of S. temminckii for shrimp, this approach identified additional, albeit yet unidentified, copepod species as being important in the diet of S. watermeyeri. We conclude that the lower number of species identified using metabarcoding was most likely a result of amplification bias, resulting in key copepod species missing from the dietary analysis. These findings suggest that metagenomics is not only a useful complementary method for molecular dietary analysis, but may in some cases outperform metabarcoding. However, metagenomics is even more strongly affected by the lack of reference sequences than is metabarcoding, as the majority of sequences originate from genomic regions that have not yet been sequenced for the putative prey species in question.